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About

Stem Cell Instrumentation Foundry

Overview of Services

The Stem Cell Instrumentation Foundry (SCIF)is a set of core research facilities which (1) Cleanroom core for micro/nanofabrication, (2) flow cytometry and cell sorting core, (3) Cell culture core.  


1) The clean room core houses instruments for nanofabrication. Listed below are the instruments and areas available. 
-Photolithography (spin coater, mask aligner, work hood for development and drying) 
-Sputtering
-FEI 800 Focused Ion Bean with deposition 
-Atomic Layer Deposition 
-ebeam
-Multiple work hoods 

2) The cytometry core in the SCIF provides advanced flow cytometry and cell sorting instrumentation, operator assistance, training, and consultation. Instruments include: 
-BD FACS Aria III cell sorter 
-Cytek Aurora - 4 Laser
-Bio-Rad ZE5 analyzer (27 colors) 
-BD LSR II analyzer (11 colors)
-For instrument specs visit: https://scif.ucmerced.edu/node/7  

3) Cell culture resources include biological safety cabinets, incubators, and associated equipment for performing cell culture. 

 

 

RESTORE is a shared resources laboratory and collaborative scientific facility that provides researchers with access to specialized equipment, expertise, training, and support. The facility is comprised of three core areas: (1) Cell Analysis, (2) Histology, and (3) Whole Organism and Specimen Scanning.
 

1) Cell Analysis Equiment:

-BD FACS Discover S8 Cell Sorter with Imaging
-Leica SP8 Inverted Confocal Microscope

2) Histology Equiment:

-Excelsior AS Tissue Processor 
-Histostar Embedding Center
-CryoStar NX50 Cryostat
-PrintMate Cassette Printer 
-SlideMate AS

3) Whole Organism and Specimen Scanning:

-Perimed PeriCam PSI laser speckle contrast imager 
-Madic Lab MicroPET Scanner
-Revvity IVIS SpectrumCT 
-Revvity Quantum GX3 MicroCT

 

Leadership

 David Gravano, PhD  Technical Director of Cytometry
 P Kumar, PhD  Technical Director of Cleanroom
 Marisa Bickel, PhD  Lab Manager
   

 

Location and hours of operation

Hours Location

 24/7 Self Service
 Monday-Friday 9AM-5PM 

BSP 412 - Cytometry
S&E1 154 - Cleanroom
BSP 018 - RESTORE

Links and Resources

  1. Stem Cell Instrumentation Foundry
  2. Stem Cell Instrumentation Foundry New User Document

Contacts

Name Role Phone Email Location
David Gravano
Technical Director of Cytometry
 
209-285-5726
 
dgravano@ucmerced.edu
 
BSP 412
 
P. Kumar
Technical Director of Cleanroom
 

 
pkumar22@ucmerced.edu
 
SE1-153
 
Marisa Bickel
Lab Manager
 
303-547-6305
 
marisabickel@ucmerced.edu
 
BSP 075