The Stem Cell Instrumentation Foundry (SCIF)is a set of core research facilities which (1) Cleanroom core for micro/nanofabrication, (2) flow cytometry and cell sorting core, (3) Cell culture core.
1) The clean room core houses instruments for nanofabrication. Listed below are the instruments and areas available.
-Photolithography (spin coater, mask aligner, work hood for development and drying)
-Sputtering
-FEI 800 Focused Ion Bean with deposition
-Atomic Layer Deposition
-ebeam
-Multiple work hoods
2) The cytometry core in the SCIF provides advanced flow cytometry and cell sorting instrumentation, operator assistance, training, and consultation. Instruments include:
-BD FACS Aria III cell sorter
-Cytek Aurora - 4 Laser
-Bio-Rad ZE5 analyzer (27 colors)
-BD LSR II analyzer (11 colors)
-For instrument specs visit: https://scif.ucmerced.edu/node/7
3) Cell culture resources include biological safety cabinets, incubators, and associated equipment for performing cell culture.
1) Cell Analysis Equiment:
-BD FACS Discover S8 Cell Sorter with Imaging
-Leica SP8 Inverted Confocal Microscope
2) Histology Equiment:
-Excelsior AS Tissue Processor
-Histostar Embedding Center
-CryoStar NX50 Cryostat
-PrintMate Cassette Printer
-SlideMate AS
3) Whole Organism and Specimen Scanning:
-Perimed PeriCam PSI laser speckle contrast imager
-Madic Lab MicroPET Scanner
-Revvity IVIS SpectrumCT
-Revvity Quantum GX3 MicroCT
| David Gravano, PhD | Technical Director of Cytometry |
| P Kumar, PhD | Technical Director of Cleanroom |
| Marisa Bickel, PhD | Lab Manager |
| Hours | Location |
|
24/7 Self Service |
BSP 412 - Cytometry |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| David Gravano |
Technical Director of Cytometry
|
209-285-5726
|
dgravano@ucmerced.edu
|
BSP 412
|
| P. Kumar |
Technical Director of Cleanroom
|
pkumar22@ucmerced.edu
|
SE1-153
|
|
| Marisa Bickel |
Lab Manager
|
303-547-6305
|
marisabickel@ucmerced.edu
|
BSP 075
|